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Gainst time. The mean values from three experiments are shown with S.E. (error bars). Circles, wild type; squares, I54A; triangles, L47Q/L55Q.FIGURE 2. Co-precipitation of 32 with DnaK and/or DnaJ. A, 32 (0.4 M) was incubated with DnaK (1.6 M) at 30 for 30 min and treated with anti- 32 serum. The precipitates formed were subjected to SDS-PAGE. 32 and DnaK were detected by CBB staining (suppleme
Known to be "promiscuous" [109] with multiple binding partners. This promiscuity most likely is due to the multiple surfaces presented by the IDPs or the low energy requirement associated with their role in mineralization. Thus, it is not surprising that DMP1 can interact with cell membrane receptors (e.g. v3 integrin [122] to regulate signaling [123], FGF23 expression [66], bind to HA crystals s
Low CNKSR1 expression (p = 0.3666). Pancreatic tumors with nuclear in addition to cytoplasmic CNKSR1 staining (32/107) showed increased nuclear phospho-ERK expression compared to tumor with cytoplasmic CNKSR1 staining only (p = 0.017). Conclusion: CNKSR1 expression is increased in pancreatic tissue specimens and was found to be an independent prognostic marker of overall survival. CNKSR1 may help
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Ibed above. With phosphorylation of extracellular-signalregulated kinase (ERK) inducing nuclear translocation staining was predominantly nuclear with a few cases also showing cytoplasmic staining. Scoring of p-ERK1/2 was done blinded to the CNKSR1 results using standard intensity scores above (0 = no staining, 1 = weak staining, 2 = moderate staining, 3 = strong staining). In addition, the percen
Orrespondence:; 3 Computational Biology Group, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa 1 Division of Medical Virology, University of Cape Town, Cape Town, South Africain west central Africa, at 5.3 [8]. This, together with the co-circulation of divergent variants of multiple clades,
Re strongly expected to have decreased affinity for DnaK and/or DnaJ chaperones. To test this hypothesis, co-immunoprecipitation experiments were performed using anti- 32 serum. Because the DnaK- 32 complex was not detected in the presence of ATP, and DnaK was precipitated even without 32 in the presence of ADP, these experiments were performed without adding nucleotide. When I54A 32 or L47Q/L55Q
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Le interaction between the altered 32 protein and DnaK. It is thus conceivable that the binding of DnaJ to wild-type 32 induces a stable interaction with DnaK. To further evaluate the extent of 32-DnaK (or 32-DnaJ) interaction, mixtures of 32, DnaK, and DnaJ were analyzed by gel filtration. DnaJ-His was used instead of DnaJ because of its higher purity. DnaK concentration was varied from 0.8 to 3
Er This property is particularly . suitable for vaccine design. There isEr This property is particularly . suitable for vaccine design. There is no limitation to the size of peptide displayed, given the ability of the E2 CD to naturally present 60 lipoyl domains plus 60 copies of the E1 (150 kDa) or E3 (100 kDa) enzymes. [61] Domingo et al demonstrated that a green fluorescentWJV|www.wjgnet.
Virus like particles (VLPs), also called pseudovirions, are composed of oneVirus like particles (VLPs), also called pseudovirions, are composed of one or more viral structural proteins (capsid and/or envelope proteins) that retain the ability to selfassemble into multimeric structures (or subviral particles) when expressed in vitro using recombinant protein expression systems - including pla
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