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A. A network time server also a NTP time server is a network device that gets a UTC time signal and after distributes it among the additional devices on a network. Period protocol NTP then suggests that all machines are kept synchronised compared to that time.



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Gainst time. The mean values from three experiments are shown with S.E. (error bars). Circles, wild type; squares, I54A; triangles, L47Q/L55Q.FIGURE 2. Co-precipitation of 32 with DnaK and/or DnaJ. A, 32 (0.4 M) was incubated with DnaK (1.6 M) at 30 for 30 min and treated with anti- 32 serum. The precipitates formed were subjected to SDS-PAGE. 32 and DnaK were detected by CBB staining (suppleme
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Ning (no staining for p-ERK (score 0); weak p-ERK (score 1+), moderate p-ERK (score 2+), and strong p-ERK (score 3 +) staining) in Fig. 3. Staining intensities were grouped as dichotomous variables, defining scores 0? as low and 2? as high expression levels [25]. Evaluation of staining was carried out independently by two pathologists (MM and MA) blinded to patients' outcome and pathological stag
Ning (no staining for p-ERK (score 0); weak p-ERK (score 1+), moderate p-ERK (score 2+), and strong p-ERK (score 3 +) staining) in Fig. 3. Staining intensities were grouped as dichotomous variables, defining scores 0? as low and 2? as high expression levels [25]. Evaluation of staining was carried out independently by two pathologists (MM and MA) blinded to patients' outcome and pathological stag
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Known to be "promiscuous" [109] with multiple binding partners. This promiscuity most likely is due to the multiple surfaces presented by the IDPs or the low energy requirement associated with their role in mineralization. Thus, it is not surprising that DMP1 can interact with cell membrane receptors (e.g. v3 integrin [122] to regulate signaling [123], FGF23 expression [66], bind to HA crystals s
Inase suppressor of Ras 1 (CNKSR1)* Correspondence: rudloffu@mail.nih.gov Equal contributors 1 Thoracic and Gastrointestinal Oncology Branch, Gastrointestinal Oncology Section, Investigator Center for Cancer Research, National Cancer Institute, Building 10 - Hatfield CRC, Room 4-5950, Bethesda, MD 20892, USA Full list of author information is available at the end of the article?The Author(s). 20
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Wild-type at all temperatures (30, 37, and 42 ) (Fig. 1 and supplemental Fig. S2). These results indicate that stability of 32 in vivo does not always reflect its direct susceptibility to proteases and that the high stability of I54A 32 in vivo is attributed to some intracellular state of 32. Stable 32 Mutants Show Reduced Affinity for DnaJ--The above results showed that other factors in additio
Low CNKSR1 expression (p = 0.3666). Pancreatic tumors with nuclear in addition to cytoplasmic CNKSR1 staining (32/107) showed increased nuclear phospho-ERK expression compared to tumor with cytoplasmic CNKSR1 staining only (p = 0.017). Conclusion: CNKSR1 expression is increased in pancreatic tissue specimens and was found to be an independent prognostic marker of overall survival. CNKSR1 may help
Le interaction between the altered 32 protein and DnaK. It is thus conceivable that the binding of DnaJ to wild-type 32 induces a stable interaction with DnaK. To further evaluate the extent of 32-DnaK (or 32-DnaJ) interaction, mixtures of 32, DnaK, and DnaJ were analyzed by gel filtration. DnaJ-His was used instead of DnaJ because of its higher purity. DnaK concentration was varied from 0.8 to 3
Le interaction between the altered 32 protein and DnaK. It is thus conceivable that the binding of DnaJ to wild-type 32 induces a stable interaction with DnaK. To further evaluate the extent of 32-DnaK (or 32-DnaJ) interaction, mixtures of 32, DnaK, and DnaJ were analyzed by gel filtration. DnaJ-His was used instead of DnaJ because of its higher purity. DnaK concentration was varied from 0.8 to 3
Major capsid protein L1 produced in S. cerevisiae. In 2009 the FDAMajor capsid protein L1 produced in S. cerevisiae. In 2009 the FDA approved Cervarix, a bivalent (HPV types 16 and 18) vaccine commercialized by GlaxoSmithKline (GSK). Both the HPV VLP vaccines have shown to have a sustained prophylactic efficacy in clinical trials against infection and genital disease, generating a long-lasti
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